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KMID : 0357119990210040369
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Korean Journal of Immunology
1999 Volume.21 No. 4 p.369 ~ p.375
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Analysis of lnteraction between Vav and TcR ζ Chain in Jurkat T Cell Activation
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김병석/Wook Jin Chae
Jin Hwan Han/Sang Won Kim/Young Sup Song/Kyung Min Cho/Myung Chull Rhee/Sang Kyou Lee
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Abstract
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When T cells are encountered with MHC (Major Histocompatibility Complex): peptide complex, they can be activated via T cell receptor complex. Among T cell receptor complex, chains have three ITAMs (Immunoreceptor Tyrosine based Activation Motifs) in their cytoplasmic tail for triggering the diverse intracellular downstream signaling cascades. To analyze the importance of each ITAM, we made stable Jurkat transfectants expressing high level of CD8- chimeric mo~lecule and its mutant forms where the tyrosine residues in each ITAM were changed into phenylalanine on their surface. Mutant transfectants showed distinct pattern of tyrosine phosphorylation upon activation including changes in tyrosine phosphorylation of GNEF (Guanine Nucleotide Exchange Factor) Vav. Among three ITAMs, A ITAM was proved to be critical role for association with Vav for activation of Jurkat stable transfectants. Mutant transfectants mutated at A ITAM (A1 and A1A2) showed remarkable reduction in phosphorylation and association of Vav with chain and other important signaling molecules including ZAP-70 and SLP-76 compared to the other transfectants. This finding suggests that Vav can deliver the downstream activation signaling via association with A ITAM directly or indirectly.
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KEYWORD
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T Cell, Activation, Vav, ITAM,
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